The present title has been designed for those having a fundamental grasp of elementary anatomy, histology, cell physiology, and the basic principles of biochemistry, but have little or no experience in tissue culture. It is intended as an introduction to the theory of the technique and biology of cultured cells as well as a practical step guide to procedures, and should be of value to any one without any, or with little, prior experience in tissue culture. The topics, vividly x-rayed are: The Cell; Cell Division (mitosis & meiosis); The cell and its Environment; Culture Media; Equipments; Prevention of Contamination; sterilization; Aseptic techniques; Special Techniques; Physical Methods of Cell Separation; Primary Explanation Techniques; Culture of Specific Cell Types; Three Dimensional Culture System; Cultivation of Cells in Vivo transplantation etc.