About the Book
After 20 years of the first publication on vitrification as an ice crystal-free cryopreservation method for embryos, there is a chronic need for a book that addresses this cryopreservation technique in detail, outlining those techniques that result in success, and providing recommended means of overcoming the problems typically encountered. The book will supply a historical overview of the principles and development of vitrification techniques in animals and humans; the various consumables utilized for the vitrification of cells and tissue and suppliers thereof; and the vitrification and warming technique for oocytes, sperm, pronuclear stage embryos, cleavage stage embryos, blastocysts, ovarian tissue, and embryonic stem cells. Wherever applicable, the set-up including protocol, operation, maintenance, and trouble-shooting of the different protocols used for vitrifying various stages of cells and tissues will also be covered.
Cryopreservation methods have become well established as an increasingly important therapeutic strategy in assisted reproduction. Vitrification in Assisted Reproduction: A User's Manual and Trouble-shooting Guide addresses this cryopreservation technique from an academic and technical viewpoint, with specific reference to assisted reproduction in humans. Hence, two decades after the first publication on vitrification as an ice crystal-free cryopreservation method for embryos, this book fulfills the need for a book that considers this new and under-utilized cryopreservation technique in detail, outlining those techniques that result in success, and providing recommended means of overcoming the problems typically encountered.
The book covers a historical overview of the principles and development of vitrification techniques in animal and humans, the various consumables utilized for the vitrification of cells and tissue and suppliers thereof; the vitrification and warming technique for oocytes, sperm, pronuclear stage embryos, cleavage stage embryos, blastocysts, ovarian tissue and embryonic stem cells. Wherever applicable, the setting up including protocol, operation, maintenance and trouble-shooting of the different protocols used for vitrifying various stages of cells and tissues is also covered.
Vitrification in Assisted Reproduction is an invaluable textbook for clinical and reproductive medicine practitioners and ART specialists.